摘要
目的 探讨滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶中的代谢规律。方法 采用超高效液相色谱法(UPLC)分别测定滇重楼不同居群间、同一居群不同植株间根茎、茎、叶中甾体皂苷活性成分的含量,并建立其UPLC图谱,对滇重楼不同居群间、同一居群不同植株间甾体皂苷活性成分在根茎、茎、叶的代谢规律进行研究。结果 滇重楼不同居群间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量(P＜0.01)具有极显著差异,UPLC图谱中色谱峰个数为叶＞根茎＞茎;滇重楼同一居群不同植株间根茎、茎、叶中次生代谢产物重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ及总皂苷含量(P＜0.05)差异较小,UPLC图谱中色谱峰1、2、4、5在根茎、茎、叶均存在,但叶比根茎、茎分别还多11、16个色谱峰。结论 滇重楼不同居群间、同一居群不同植株的茎中主要活性成分含量较低、种类较少;滇重楼甾体皂苷活性成分在同一居群不同植株间根茎、茎、叶中的代谢具有较稳定的居群遗传能力,在不同居群间的代谢存在较大的差异;滇重楼茎、叶是否可以作为其根茎的替代品入药,仍需进一步做药理、毒理及临床实验。
Abstract
OBJECTIVE To discuss the metabolism rule of the active steroidal saponins in the roots, stems and leaves of Paris polyphylla var. yunnanensis Franch belonging to different populations and different plants in the same population. METHODS UPLC was used to determine the contents of the active ingredients including polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ, and polyphyllin Ⅶ from the roots, stems, and leaves, the UPLC map was established, the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A) developed by the Chinese Pharmacopoeia Committee was used to analyze the number of peaks in the UPLC map, and One-way ANOVA was used to analyze their metabolism rule. RESULTS There was significant difference in the contents of polyphyllin Ⅰ, polyphyllin Ⅱ, polyphyllin Ⅵ, polyphyllin Ⅶ, and their total contents in the roots, stems, and leaves among different populations (P<0.01). The number of peaks in the UPLC map ranked in the folloing order:leaf> root> stem. The difference among different plants of the same Paris polyphylla var. yunnanensis Franch population was smaller. No. 1, 2, 4, 5 peaks all existed in the UPLC maps of the roots, stems, and leaves, but there were 11 and 16 more peaks in the leaves than the roots and stems, respectively. CONCLUSION There are fewer kinds of main active ingredients with low contents in the stems of Paris polyphylla var. yunnanensis Franch. The population genetic capabilities of the metabolism of the steroidal saponins active ingredients are stable in the roots, stems and leaves of different plants from the same population, but there are large differences between different populations. Further pharmacological, toxicological, and clinical studies need to be done to determine whether the leaves and stems could take the place of root as an alternative medicine.
关键词
滇重楼 /
超高效液相色谱法 /
次生代谢产物
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Key words
Paris polyphylla var. yunnanensis Franch. /
ultra performance liquid chromatography /
secondary metabolite
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冯丽丽,张琳,李海峰,,*,张成桂.
滇重楼次生代谢产物甾体皂苷活性成分代谢规律的研究[J]. 中国药学杂志, 2015, 50(8): 664-670 https://doi.org/10.11669/cpj.2015.08.003
FENG Li-li, ZHANG Lin, LI Hai-feng, ,*, ZHANG Cheng-gui.
Metabolism Rule of Secondary Metabolites, Steroidal Saponins Active Ingredients, in Paris polyphylla var. yunnanensis Franch. [J]. Chinese Pharmaceutical Journal, 2015, 50(8): 664-670 https://doi.org/10.11669/cpj.2015.08.003
中图分类号：
Q945
R284
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参考文献
[1] LI H. Plants of the Genus Polyphylla (重楼属植物). Beijing:Science Publishing Company, 1998:118. [2] LI P, FU J H, WANG J K, et al. Extract of Paris polyphylla Simth protects cardiomyocytes from anoxia-reoxia injury through inhibition of calcium overload. Chin J Integr Med(中国结合医学杂志), 2011, 17(4):283-289. [3] YE F, YANG G Y, LI Z R, et al. Extraction process and quality control of Polyphyllins . China Pharm(中国药师), 2011, 14(8):1207-1209. [4] ZHEN W M, ZHAO T Z. Preliminary study on the optimal pollination period of Paris polyphylla var. yunnanensis . Med Plant(药用植物研究), 2012, 3:8-10. [5] HE M S, LI X. Progress of pharmacological research on paridis . World Chin Med(世界中医药杂志), 2012, 31(7):579-582. [6] LIU Y H, YANG X, LI J L, et al. The polyketide metabolites from the endophytic fungus Penicillium sp. (No. 4) of Paris polyphylla Sm . Nat Prod Res Dev(天然产物研究与开发), 2013, 25:431-434,493. [7] LIU X, CHEN Y H, WU Z Y, et al. Paris polyphylla market demand and price analysis . Mod Chin Med(中国现代中药), 2007, 9( 6):451. [8] Ch. P(2010) Vol Ⅰ(中国药典2010年版.一部) . 2010:243-244.[9] ZENG W M, ZHAO T Z. Analysis on utilization of stems and leaves growing on the ground of Paris polyphylla var. yunnanensis . Chin Agricul Sci Bull(中国农学通报), 2012, 8(18):266-270. [10] NIAN S H, LIU L M. Initial development and experimental study of the aerial parts of Paridis . Yunnan J Tradit Chin Med Mater Med(云南中医中药杂志),2007, 28(4):35-36.
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脚注
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基金
国家自然科学基金资助项目(81360616);云南省自然科学基金资助项目(2010ZC136);云南省教育厅基金重点资助项目(2013Z153)
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